Molecular Techniques in Detection of Triazole Resistance Gene Mutations in Common Pathogenic Fungi



Khodavaisy S¹ ; Mahmoudi S¹ ; Afshari SAK¹ ; Salehi Z² ; Kord M¹ ; Badali H³
Source: Journal of Mazandaran University of Medical Sciences Published:2017

Abstract

Invasive candidiasis and aspergillosis are amongst major medical concerns with high mortality and morbidity in immunocompromised patients. Management of these infections is dependent on early and efficient antifungal therapy, as well as drug resistance monitoring. Decreased sensitivity of these pathogens to antifungal drugs during recent decades, rapid detection of drug resistance in pathogenic fungi is highly recomended. Generally, identification of drug resistance associated mutations requires complicated and expensive methods such as DNA sequencing. However, nowadays application of accurate, fast and highly sensitive techniques, including Rolling Circle Amplification (RCA), PCR-RFLP, Real-Time PCR, and ARMS-PCR provide the possibility for detection of target sequence containing nucleotide polymorphisms even at the one base pair level. In this review we aimed to discuss the usage, advantages and disadvantages of these techniques in order to identify the mutations of the azole-resistant strains. © 2017, Mazandaran University of Medical Sciences. All rights reserved.