Cytotoxic Effects of Hyperthermia, Chemotherapy (Navelbine) and Radiation on Glioma Spheroids Publisher



Mahdavi SR¹ ; Yahyapour R² ; Nikoofar A³
Source: Radiation Physics and Chemistry Published:2016

Abstract

Introduction: Glioblastoma multiform (GBM) is the most common malignant primary brain tumor in humans. Current conventional treatment could not effectively increase the survival rate of patients who suffered from GBM. In clinical view, some experiments which can mimic in vivo conditions to evaluate anticancer agent effects on tumors are very useful. We can fairly reach to this goal using 3-dimentional spheroids rather than monolayer culture. The aim of this study was to determine the cytotoxicity caused by Navelbine, hyperthermia and radiation on glioma spheroids. Methods: The spheroid cells were separately or simultaneously treated with the doses of 7.5 μg/ml Navelbine for 50.3 h, irradiated with 2 Gy and incubated at 43 °C for 1 h. Comet and colony formation assays were applied to assess the DNA damages and survival rate of the cells. Results: In both comet and colony formation assays, cell damage in all treated groups was significantly higher in comparison with control group (P<0.05). Moreover, the synergistic effect of combined groups such as: hyperthermia and radiotherapy, Navelbine and radiotherapy and combination of Navelbine-hyperthermia-radiotherapy was observed. Conclusions: Combined agents with low concentration can cause a synergistic effect on treatment procedure and increased damages in multicellular spheroids. © 2016 Elsevier Ltd.