Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice

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Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice Publisher Pubmed

Sabet FS¹ ; Hosseini M^{1, 2} ; Khabbaz H¹ ; Dadmehr M³ ; Ganjali MR^{4, 5}

Source: Food Chemistry Published:2017

Abstract

Aflatoxins are potential food pollutants produced by fungi. Among them, Aflatoxin B1 (AFB1) is the most toxic. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, utilizing a FRET-based method, we have developed a nanobiosensor for detection of AFB1 in agricultural foods. Aptamer-conjugated Quantum dots (QDs) are adsorbed to Au nanoparticles (AuNPs) due to interaction of aptamers with AuNPs leading to quenching effect on QDs fluorescence. Upon the addition of AFB1, the specific aptamers are attracted to AFB1, getting distance from AuNPs which result in fluorescence recovery. Under optimized conditions the detection limit of proposed nanobiosensor was 3.4 nM with linear range of 10–400 nM. Selectivity test demonstrates that the nanobiosensor could be a promising tool for specific evaluation of food stuff. This method was successfully applied for the analysis of AFB1 in rice and peanut samples. © 2016 Elsevier Ltd

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Style	Citing Format
MLA	Sabet FS, et al.. "Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice." Food Chemistry, vol. 220, no. , 2017, pp. 527-532.
APA	Sabet FS, Hosseini M, Khabbaz H, Dadmehr M, Ganjali MR (2017). Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice. Food Chemistry, 220(), 527-532.
Chicago	Sabet FS, Hosseini M, Khabbaz H, Dadmehr M, Ganjali MR. "Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice." Food Chemistry 220, no. (2017): 527-532.
Harvard	Sabet FS et al. (2017) 'Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice', Food Chemistry, 220(), pp. 527-532.
Vancouver	Sabet FS, Hosseini M, Khabbaz H, Dadmehr M, Ganjali MR. Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice. Food Chemistry. 2017;220():527-532.
BibTex	@article{ author = {Sabet FS and Hosseini M and Khabbaz H and Dadmehr M and Ganjali MR}, title = {Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice}, journal = {Food Chemistry}, volume = {220}, number = {}, pages = {527-532}, year = {2017} }
RIS	TY - JOUR AU - Sabet FS AU - Hosseini M AU - Khabbaz H AU - Dadmehr M AU - Ganjali MR TI - Fret-Based Aptamer Biosensor for Selective and Sensitive Detection of Aflatoxin B1 in Peanut and Rice JO - Food Chemistry VL - 220 IS - SP - 527 EP - 532 PY - 2017 ER -

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