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First Molecular Report of Causative Agent of Otomycosis Due to Aspergillus Luchuensis Publisher Pubmed



Sarvestani HK1 ; Ghazvini RD1 ; Hashemi SJ1 ; Rezaie S1 ; Mahmoudi S2 ; Shoar MG1 ; Elahi M3 ; Izadi A1 ; Getso MI1, 4 ; Rafat Z5 ; Tajdini A3 ; Hedayati MT6
Authors

Source: Journal of Wound Care Published:2021


Abstract

Otomycosis is a fungal infection of the external auditory canal caused mainly by the genus Aspergillus. Aspergillus luchuensis, an industrially important fungus, is a member of Aspergillus section Nigri. In this report, we present a case of otomycosis due to Aspergillus luchuensis in a 43-year-old female patient. We performed a partial PCRsequencing of β-tubulin and calmodulin genes to identify the isolate to the species level. Further, we determined the in vitro susceptibility of the isolate to nystatin, clotrimazole and itraconazole according to the Clinical & Laboratory Standards Institute (CLSI) M38-A2 protocol. Accordingly, the minimum inhibitory concentrations of clotrimazole, nystatin and itraconazole were 0.25μg/mL, 0.5μg/mL and 1μg/mL, respectively. This is the first report of clinically relevant isolation of Aspergillus luchuensis identified by a molecular technique as a causative agent of otomycosis. © 2020 MA Healthcare ltd.
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