Combination of Interleukin-10 Gene Promoter Polymorphisms With Hla-Drb1*15 Allele Is Associated With Multiple Sclerosis Publisher Pubmed



Shahbazi M¹ ; Abadi JSA¹ ; Roshandel D¹ ; Koochaki M¹ ; Amiri H^{1, 2} ; Kohansal R^{1, 2} ; Baghbanian SM³ ; Zamani M⁴
Source: Indian Journal of Medical Research Published:2017

Abstract

Background & objectives: Multiple sclerosis (MS) is common in some ethnic groups. Interleukin-10 (IL-10) is a potent anti-inflammatory and immunosuppressive cytokine that may be an important regulator in MS disease pathogenesis. IL-10 promoter includes several single nucleotide polymorphisms and the level of IL-10 expression is related to these polymorphisms. Furthermore, loci within the histocompatibility regions are responsible for susceptibility to MS. The aim of this study was to investigate the association of IL-10 gene promoter polymorphisms and HLA-DRB1*15 allele frequencies with MS susceptibility in an Iranian population. Methods: In this study 336 MS patients and 454 healthy controls were included. Genomic DNA was purified from peripheral blood samples by a standard protocol. Genotyping was performed by the sequence-specific primer polymerase chain reaction method. Results: IL-10 −1082 G/G and IL-10 −819 C/C genotypes were more frequent in MS patients than healthy individuals. DRB1*15 allele showed a higher frequency among MS patients compared to controls. Interpretation & conclusions: The IL-10 and HLA-DRB1*15 polymorphisms were associated with the susceptibility to MS in Iranian patients. Our results suggest that gene-gene interaction of IL-10 polymorphisms and HLA-DRB1*15 alleles may be important factors in the development of MS. © 2017, Indian Council of Medical Research. All rights reserved.