Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation Publisher Pubmed



Hajiramezanali M1 ; Atyabi F2, 3 ; Mosayebnia M1 ; Akhlaghi M4 ; Geramifar P4 ; Jalilian AR4 ; Mazidi SM5 ; Yousefnia H6 ; Shahhosseini S7 ; Beiki D4
Authors

Source: International Journal of Nanomedicine Published:2019


Abstract

Introduction: Nowadays, nanoparticles (NPs) have attracted much attention in biomedical imaging due to their unique magnetic and optical characteristics. Superparamagnetic iron oxide nanoparticles (SPIONs) are the prosperous group of NPs with the capability to apply as magnetic resonance imaging (MRI) contrast agents. Radiolabeling of targeted SPIONs with positron emitters can develop dual positron emission tomography (PET)/MRI agents to achieve better diagnosis of clinical conditions. Methods: In this work, N,N,N-trimethyl chitosan (TMC)-coated magnetic nanoparticles (MNPs) conjugated to S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (DOTA) as a radioisotope chelator and bombesin (BN) as a targeting peptide (DOTA-BN- TMC-MNPs) were prepared and validated using fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and powder X-ray diffraction (PXRD) tests. Final NPs were radiolabeled with gallium-68 ( 68 Ga) and evaluated in vitro and in vivo as a potential PET/MRI probe for breast cancer (BC) detection. Results: The DOTA-BN-TMC-MNPs with a particle size between 20 and 30 nm were efficiently labeled with 68 Ga (radiochemical purity higher than 98% using thin layer chromatography (TLC)). The radiolabeled NPs showed insignificant toxicity (>74% cell viability) and high affinity (IC 50 =8.79 µg/mL) for the gastrin-releasing peptide (GRP)-avid BC T-47D cells using competitive binding assay against 99m Tc-hydrazinonicotinamide (HYNIC)-gamma-aminobutyric acid (GABA)-BN (7-14). PET and MRI showed visible uptake of NPs by T-47D tumors in xenograft mouse models. Conclusion: 68 Ga-DOTA-BN-TMC-MNPs could be a potential diagnostic probe to detect BC using PET/MRI technique. © 2019 Hajiramezanali et al.
Related Docs
View other Related Docs
1. Exploring Innovative Strides in Radiolabeled Nanoparticle Progress for Multimodality Cancer Imaging and Theranostic Applications, Cancer Imaging (2024)
2. Development of Ga-68 Labeled, Biotinylated Thiosemicarbazone Dextran-Coated Iron Oxide Nanoparticles As Multimodal Pet/Mri Probe, International Journal of Biological Macromolecules (2020)
3. Dual Nano-Sized Contrast Agents in Pet/Mri: A Systematic Review, Contrast Media and Molecular Imaging (2016)
Experts (# of related papers)
View all Related Experts
Rassoul Dinarvand (3)
Fatemeh Atyabi (2)
Other Related Docs
4. Crucial Role of the Protein Corona for the Specific Targeting of Nanoparticles, Nanomedicine (2015)
5. Silencing of Il-6 and Stat3 by Sirna Loaded Hyaluronate-N,N,N-Trimethyl Chitosan Nanoparticles Potently Reduces Cancer Cell Progression, International Journal of Biological Macromolecules (2020)
6. Biodistribution Evaluation of 166Ho–Dtpa–Spion in Normal Rats, Journal of Radioanalytical and Nuclear Chemistry (2016)
7. Development of Radiolabeled Dextran Coated Iron Oxide Nanoparticles With 111In and Its Biodistribution Studies, International Journal of Radiation Research (2020)
8. A New Theranostic Complex Based on Bismuth-Iron Oxide Nanoparticle for Myocardial Ischemia: In Vitro Study, Nanomedicine Research Journal (2022)
9. Dendrimer Functionalized Magnetic Nanoparticles As a Promising Platform for Localized Hyperthermia and Magnetic Resonance Imaging Diagnosis, Journal of Cellular Physiology (2019)
10. Ph-Responsive Polymer in a Core–Shell Magnetic Structure As an Efficient Carrier for Delivery of Doxorubicin to Tumor Cells, International Journal of Polymeric Materials and Polymeric Biomaterials (2018)
11. Theranostic Muc-1 Aptamer Targeted Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Magnetic Resonance Imaging and Photothermal Therapy of Colon Cancer, Colloids and Surfaces B: Biointerfaces (2016)
12. Theranostic Rgd@Fe3o4-Au/Gd Nps for the Targeted Radiotherapy and Mr Imaging of Breast Cancer, Cancer Nanotechnology (2023)
13. Gd-Based Nanoparticles Mediated Magnetic Field Enhancement Inside Homogenous Tissue: Simulation Using Finite Element Method, IFMBE Proceedings (2015)
14. Magnetite/Dextran-Functionalized Graphene Oxide Nanosheets for in Vivo Positive Contrast Magnetic Resonance Imaging, RSC Advances (2015)
15. Synthesis, Characterization and Mri Application of Cobalt-Zinc Ferrite Nanoparticles Coated With Dmsa: An In-Vivo Study, Applied Magnetic Resonance (2021)
16. Biomedical Applications of Superparamagnetic Nanoparticles in Molecular Scale, Current Organic Chemistry (2015)
17. Teriflunomide Loaded Spion Nanoparticles Induced Apoptosis in Mda-Mb-231 Breast Cancer Cells, Journal of Cluster Science (2023)
18. The Effect of Superparamagnetic Iron Oxide Nanoparticles Surface Engineering on Relaxivity of Magnetoliposome, Contrast Media and Molecular Imaging (2016)
19. Design, Characterization and Green Synthesis of Samarium-Decorated Magnetic Fe3o4 Nanoparticles: Cytotoxicity and Dna Binding Studies, Journal of Biomolecular Structure and Dynamics (2023)
20. Folic Acid-Conjugated Fe-Au-Based Nanoparticles for Dual Detection of Breast Cancer Cells by Magnetic Resonance Imaging and Computed Tomography, Frontiers in Biomedical Technologies (2024)