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Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa) Publisher Pubmed



Salmaninejad A1 ; Estiar MA1 ; Gill RK2 ; Shih JH3 ; Hewitt S4 ; Jeon HS5 ; Fukuoka J6 ; Shilo K7 ; Shakoori A1, 2, 3 ; Jen J8
Authors

Source: Clinical Laboratory Published:2015


Abstract

Background: Immunohistochemical analysis (IHC) of tissue microarray (TMA) slides enables large sets of tissue samples to be analyzed simultaneously on a single slide. However, manual evaluation of small cores on a TMA slide is time consuming and error prone. Methods: We describe a computer aided scoring and analysis (CASA) method to allow facile and reliable scoring of IHC staining using TMA containing 300 non-small cell lung cancer (NSCLC) cases. In the two previous published papers utilizing our TMA slides of lung cancer we examined 18 proteins involved in the chromatin machinery. We developed our study using more proteins of the chromatin complex and several transcription factors that facilitate the chromatin machinery. Then, a total of 78 antibodies were evaluated by CASA to derive a normalized intensity value that correlated with the overall staining status of the targeting protein. The intensity values for TMA cores were then examined for association to clinical variables and predictive significance individually and with other factors. Results: Using our TMA, the intensity of several protein pairs were significantly correlated with an increased risk of death in NSCLC. These included c-Myc with p16, mSin3A with p16 and c-Myc with mSin A. Predictive values of these pairs remained significant when evaluated based on standard IHC scores. Conclusions: Our results demonstrate the usefulness of CASA as a valuable tool for systematic assessment of TMA slides to identify potential predictive biomarkers using a large set of primary human tissues.
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