Biomacromolecule Chitosan Carrying Meglumine Antimoniate Coated on a Silver/Polyurethane Nanocomposite As a Wound Dressing: Therapeutic Efficacy on Cutaneous Leishmaniasis Caused by Leishmania Major in Balb/C Mice Publisher Pubmed



Mohebali F¹ ; Aghabarari B¹ ; Vaezi MR¹ ; Zarei Z^{2, 3} ; Hassanpour G⁴ ; Alizadeh Z² ; Latifi A² ; Mohebali M^{2, 4}
Source: International Journal of Biological Macromolecules Published:2025

Abstract

The high drug-carrying capacity and biocompatibility of chitosan (CS), a versatile biomacromolecule, have received special attention in recent years. This study focused on CS containing meglumine antimoniate (MA) for treating leishmaniasis, which was coated onto a silver/polyurethane (Ag.MA.CS/PUF). The newly synthesized nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR), inductively coupled plasma (ICP), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) surface area analysis, field emission scanning electron microscope/energy dispersive spectroscopy (FESEM/EDS), and transmission electron microscopy (TEM). To confirm the in vivo results, we administered the Ag.MA.CS/PUF nanocomposite topically to skin lesions caused by L. major (MRHO/IR/75/ER) in 56 inbred BALB/c mice in intervention (n = 42) and control (n = 14) groups, once daily for four weeks. Skin lesion sizes and amastigote counts were measured before treatment and four weeks post-treatment. At these intervals, the average size of skin lesions in the Ag.MA.CS/PUF group decreased by 28 %, from 3.02 ± 0.98 to 2.17 ± 0.33 mm2. In contrast, the average size of lesions in the negative control group significantly increased from 3.58 ± 2.05 to 8.73 ± 5.15 mm2 (p < 0.05). Furthermore, the parasite load in the Ag.MA.CS/PUF nanocomposite group was significantly reduced by 80 % compared to the negative control group (p = 0.001). These findings suggest promising prospects for improving treatment outcomes in the future. © 2025 Elsevier B.V.