Bioengineered 3D Porous Silk Fibroin/Decellularized Human Placenta Incorporating Elastin-Like Polypeptides (Elp) Scaffold for Accelerated Wound Healing: In-Vitro and In-Vivo Studies Publisher



S Mousazadeh SEPIDEH ; A Mehrabi AREZOU ; A Mollafilabi AZAM ; NULL ; J Kiani JAFAR
Source: Journal of Drug Delivery Science and Technology Published:2025

Abstract

Introduction: Full-thickness skin injuries remain a notable challenge in clinical care. Here, we bioengineered a porous scaffold that incorporates silk fibroin (SF), decellularized human placenta (hPlacenta), and recombinant elastin-like peptide (ELP) for wound healing applications. Method &; material: Histological analysis was employed to verify the lack of cells in the hPlacenta. SDS-PAGE analysis was used to confirm the successful purification of ELPs from bacterial lysate. The porous hybrid SF/hPlacenta/ELP scaffolds were then successfully synthesized by lyophilization, and their physicochemical and biological characteristics were investigated for skin tissue engineering. The therapeutic impact of the SF/hPlacenta scaffold, either alone or in combination with ELP, was assessed in a full-thickness wound model. Result: Microscopic and spectroscopic analysis validates the formation of SF and hPlacenta. SDS-PAGE analysis confirmed the successful purification of ELPs from bacterial lysate. The introduction of 5 % ELP significantly improved the SF/hPlacenta scaffold's mechanical strength, stability, cell adhesion, and water retention. In vitro analyses confirmed enhanced cell proliferation and infiltration, while in vivo mouse models demonstrated superior hemostatic properties, accelerated wound closure, angiogenesis, and collagen deposition with the 5 % ELP scaffold. Conclusion: These findings suggest that the SF/hPlacenta/5 %ELP scaffold holds significant promise for clinical applications in wound healing and tissue regeneration. © 2025 Elsevier B.V., All rights reserved.