Synthesis of C-Dot by Hydrothermal Method and Evaluation of Its Anti-Bacterial Effect Against Antibiotic Resistant S. Areus and K. Pneumonea Publisher



Pajavand H¹ ; Mobarez AM¹ ; Nikkhah M² ; Abiri R³ ; Delnavazi MR⁴
Source: Nanomedicine Research Journal Published:2023

Abstract

Objective(s): Carbon dots (C-dots) are an emerging class of engineered nanomaterials with broad applications in medicine, bio-imaging, sensing, electronic devices, and catalysis. The study aimed to synthesize carbon nanoparticles with antibacterial therapeutic properties against clindamycinresistant Staphylococcus aureus and ciprofloxacin-resistant Klebsiella pneumonia strains. Methods: The C-dots were prepared by a hydrothermal method. Then the synthesized carbon dot were characterized by UV-visible spectroscopy, dynamic light scattering, Fourier transform infrared spectroscopy and transmission electron microscopy. The minimum inhibitory concentration of C-dots was evaluated by the micro-broth dilution method. Antibiotic susceptibility testing was performed using the disk diffusion method. Results: The C-dots significantly reduced S. aureus and K. pneumoniae strains growth when compared to untreated bacteria (control; P < 0.05). Therefore, the minimum inhibitory concentration (MIC) of C-dots for clindamycin-resistant S. areus and ciprofloxacin-resistant K. pneumoniae strains were 500 and 250 μg/ ml, respectively. The survival percentage of S. areus and K. pneumoniae decreased to 48.05% and 11.6% respectively after treatment with 250 μg/ml C-dots. However, the viability of bacteria decreased to 3.8% and 2.5% at the concentration of 500 μg/ml. Conclusions: The results show that by producing antibacterial drugs at the nanoscale, C-dots are a promising new approach to improve the effectiveness of treating infections caused by antibiotic-resistant bacterial strains. © 2023 Authors. All rights reserved.