Ag/Zno Nanoparticles As Novel Antibacterial Agent Against of Escherichia Coli Infection, in Vitro & in Vivo Publisher



Khoshkbejari M¹ ; Jafari A² ; Safari M³
Source: Oriental Journal of Chemistry Published:2015

Abstract

Urinary tract infection has change a basic challenge in human population. Antibacterial nanoparticles have turned over the new leaf in scientist's research.Ag/ZnOnanoparticleis synthesis in high temperature (5000C).FT-IR, XRD, SEM and TEM were used for determination of spectroscopic, structural and morphology of samples, respectively. Also the nanoparticlewas digested and analyzed by ICP-AES for determining the presence of residual chemical element in the nanoparticle. Bacterial sensitivity to nanoparticlewas commonly tested using by MIC and MBC tests. For achievement of in vivo tests, the renal of rats were injectedand then were infected with E.coli. After that, animals were divided to 9 treatment groupand the effect of three concentrations of MICandMBC; also intermediate concentrations of MICandMBC and each as a separate group, were studied.The particles size was less of 12nm, approximately. The MIC and MBC observed for Ag/ZnOwere 32μgml-1 and 512μgml-1 for E.coli, respectively.Treatment with a concentration 512μgml-1 ofAg/ZnO nanoparticle in combination with Ciprofloxacin reduced severity of renal cortical thickness of pyelonephritis rats.The microscopic pathological examination indicated that there was no any severe Inflammation of renal tubules in the groups that had been treated with512μgml-1 concentration of Ag/ZnO nanoparticlein combination with ciprofloxacin.In compared with the control groups.This study showed thatthe Ag/ZnO nanoparticle in cooperation with ciprofloxacin has great antimicrobial effect againstE.coli on renal damage of Pyelonephritis.