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The Effect of Il-22 and Il-28 in Induction of Type 1 Regulatory T (Tr1) Cells Pubmed



Arasteh J1 ; Ebtekar M2 ; Pourpak Z3 ; Pourfatollah AA2 ; Hassan ZM2 ; Kardar GA3 ; Zare A3 ; Saghafi S3 ; Hassan ATM4
Authors

Source: Iranian Journal of Allergy# Asthma and Immunology Published:2015


Abstract

Cytokines have been introduced as critical inducers in the development of Th subpopulations.Cytokines like IL-10 are involved in inducing regulatory T cells such as Type 1 regulatory T (Tr1) cells cells. IL-22 is a member of IL-10 family of cytokines, and IL-28A is a member of IFN-γ family. In this study, cord blood mononuclear cells (CBMC) from normal healthy individuals were isolated by Ficoll and then naive T cells were purified by CD4+CD25+ Regulatory T cell Isolation kit. The effect of these two cytokines on production of IL-5, TGF-β, IL-10, IL-4 and IFN-γ cytokines from cord blood T cells was investigated to identify Tr1 cells as well as Th1 and Th2 polarization. Flow cytometric analysis showed that IL-28A and IL-22 were not effective in expression of IL-5 and TGF-β either alone or in synergy, but in view of IL-10, IL-4 and IFN-γ, the results showed that IL-22 increased IL-10 and IL-4 but had a decreasing effect on IFN-γ. The results showed that IL-28A was not effective in increasing or decreasing the level of IL-10, IL-4 and IFN-γ. Therefore, according to these results, IL-22 and IL-28A were not effective in inducing Tr1 cells. Copyright © Spring 2015, Iran J Allergy Asthma Immunol. All rights reserved.
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