Impact of Various Culture Conditions on Ex Vivo Expansion of Polyclonal T Cells for Adoptive Immunotherapy Publisher Pubmed



Ghaffari S^{1, 2} ; Torabirahvar M^{1, 3} ; Omidkhoda A⁴ ; Ahmadbeigi N¹
Source: APMIS Published:2019

Abstract

Currently, adoptive immunotherapy is considered as one of the leading treatments in cancer. Successful adoptive immunotherapy depends on producing large numbers of desired T cells ex vivo for infusion. This requires an effective protocol for maximum functional T-cell expansion while keeping the time and costs to a minimum. Current T-cell expansion protocols are diverse in their methodology, and a universal protocol of expansion is wanting. Also, new findings regarding T-cell biology, signaling, and activation have reshaped the strategies of T-cell propagation over the years, introducing new ways to expand T cells. Here, we reviewed different conditions for blood-derived polyclonal T-cell expansion so as to elucidate the influential factors of T-cell expansion and their efficacy. © 2019 APMIS. Published by John Wiley & Sons Ltd