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Visible Light-Responsive Vanadium-Based Metal–Organic Framework Supported Pepsin With High Oxidase Mimic Activity for Food Spoilage Monitoring Publisher Pubmed



Sharifnezhad AH1 ; Dashtian K1 ; Zaredorabei R1 ; Mahdavi M2
Authors

Source: Microchimica Acta Published:2022


Abstract

A photo-induced metal–organic framework-enzyme hybrid nanosystem was developed via a controllable physical embedding method that displays dual enzymatic and photo-non-enzymatic strategy which cause high stability and cascade catalytic performance to oxidation of o-phenylenediamine and generate a UV–Vis signal at 450 nm for the tracing and sensitive detection of putrescine (Put). Under optimal conditions, the present bioassay provides a wide detection range from 0.02 to 10 µM and 20–80 µM with a detection limit of 5.5 nM, which is more desirable than numerous previous reports. In addition, the established colorimetric photo-bioassay can selectively and accurately identify Put in the presence of other distributing species. The present work provides an elegant strategy to merged photo-nanozymes’ and enzyme capabilities and also broadened the sensing strategies of photo-nanozymes with promising potential in the realm of cancer diagnosis and food quality monitoring as well as its potential in various bioassays and heterogeneous catalysis fields. Graphical Abstract: [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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