Identification of a Novel Human Rhinovirus C Type by Antibody Capture Vidisca-454 Publisher Pubmed



Farsani SMJ^{1, 2} ; Munnink BBO¹ ; Canuti M^{1, 5} ; Deijs M¹ ; Cotten M³ ; Jebbink MF¹ ; Verhoeven J^{1, 5} ; Kellam P³ ; Loens K⁴ ; Goossens H⁴ ; Ieven M⁴ ; Van Der Hoek L¹
Source: Viruses Published:2015

Abstract

Causative agents for more than 30 percent of respiratory infections remain unidentified, suggesting that unknown respiratory pathogens might be involved. In this study, antibody capture VIDISCA-454 (virus discovery cDNA-AFLP combined with Roche 454 high-throughput sequencing) resulted in the discovery of a novel type of rhinovirus C (RV-C). The virus has an RNA genome of at least 7054 nt and carries the characteristics of rhinovirus C species. The gene encoding viral protein 1, which is used for typing, has only 81% nucleotide sequence identity with the closest known RV-C type, and, therefore, the virus represents the first member of a novel type, named RV-C54. © 2015 by the authors; licensee MDPI, Basel, Switzerland.