Platelet Transfusion Outcome and Flow Cytometric Monocyte Phagocytic Assay (Fmpa) Pubmed



Sayyadi M¹ ; Shaiegan M² ; Zarif MN² ; Vaezi M³ ; Mohammadi S⁴
Source: Archives of Iranian Medicine Published:2016

Abstract

Background: This study was designed to evaluate platelet transfusion outcome via flow cytometric monocyte phagocytic assay (FMPA). Method: Fifteen patients with a history of multiple platelet transfusions and fifteen controls were enrolled in this study. CMFDA-labeled platelets were incubated with patients’ sera and were Anally incubated with monocytes in a tube and analyzed by flow cytometry. Monocytes that phagocytosed platelets were detected as a CMFDA-positive platelet population via monocyte gate. The FMPA results were compared with CCI results for the patients. Result: The FMPA result correlated with 1 -hour (r = -0.885, P = 0.001) and 24-hour (r = -0.884, P = 0.001) CCI. There is a significant difference in means of FMPA results between the patients with immune platelet refractoriness (68.46 ± 10.4%), non-refractory group (37.73 ± 15.21%) and the control group (18.27 ± 2.86%). Conclusion: Our data showed that FMPA has good results in evaluation of platelet transfusion outcome and may be useful as an indicator of platelet transfusion response. © 2016, Academy of Medical Sciences of the I.R. Iran.