Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain

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Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain Publisher Pubmed

Asgarianomran H^{1, 2} ; Amirzargar AA³ ; Zeerleder S^{4, 5} ; Mahdavi M⁶ ; Van Mierlo G⁴ ; Solati S⁴ ; Jedditehrani M⁶ ; Rabbani H⁶ ; Aarden L³ ; Shokri F^{2, 6}

Source: APMIS Published:2015

Abstract

Filamentous hemagglutinin (FHA) is a major adhesion and virulence factor of Bordetella pertussis and also a main component of acellular pertussis vaccines. Interaction of FHA with different receptors on human epithelial and immune cells facilitates entrance and colonization of bacteria as well as immunomodulation of the host immune response. Three overlapping segments of the FHA gene were cloned in a prokaryotic expression vector and the recombinant proteins were purified. These recombinant fragments along with the native FHA protein were employed to assess their potential Toll-like receptor (TLR) stimulatory effects and to localize the TLR binding region. TLR stimulation was monitored by applying HEK293-Blue cell lines cotransfected with TLR2, 4, or 5 and a NF-κB reporter gene. Culture supernatants were checked for secretion of the reporter gene product and IL-8 as indicators of TLR stimulation. Native FHA was found to strongly stimulate TLR2, but not TLR4 or TLR5 transfected cells. Among recombinant FHA fragments only the fragment spanning amino acid residues 1544-1917 was able to exhibit the TLR2 stimulating property of FHA. Interaction of FHA with TLR2 suggests its involvement in induction of the innate immune system against Bordetella pertussis. The TLR2-binding domain of FHA may contribute to immunoprotection against pertussis infection. © 2014 APMIS. Published by John Wiley & Sons Ltd.

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Style	Citing Format
MLA	Asgarianomran H, et al.. "Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain." APMIS, vol. 123, no. 2, 2015, pp. 156-162.
APA	Asgarianomran H, Amirzargar AA, Zeerleder S, Mahdavi M, Van Mierlo G, Solati S, Jedditehrani M, Rabbani H, Aarden L, Shokri F (2015). Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain. APMIS, 123(2), 156-162.
Chicago	Asgarianomran H, Amirzargar AA, Zeerleder S, Mahdavi M, Van Mierlo G, Solati S, Jedditehrani M, Rabbani H, Aarden L, Shokri F. "Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain." APMIS 123, no. 2 (2015): 156-162.
Harvard	Asgarianomran H et al. (2015) 'Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain', APMIS, 123(2), pp. 156-162.
Vancouver	Asgarianomran H, Amirzargar AA, Zeerleder S, Mahdavi M, Van Mierlo G, Solati S, et al.. Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain. APMIS. 2015;123(2):156-162.
BibTex	@article{ author = {Asgarianomran H and Amirzargar AA and Zeerleder S and Mahdavi M and Van Mierlo G and Solati S and Jedditehrani M and Rabbani H and Aarden L and Shokri F}, title = {Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain}, journal = {APMIS}, volume = {123}, number = {2}, pages = {156-162}, year = {2015} }
RIS	TY - JOUR AU - Asgarianomran H AU - Amirzargar AA AU - Zeerleder S AU - Mahdavi M AU - Van Mierlo G AU - Solati S AU - Jedditehrani M AU - Rabbani H AU - Aarden L AU - Shokri F TI - Interaction of Bordetella Pertussis Filamentous Hemagglutinin With Human Tlr2: Identification of the Tlr2-Binding Domain JO - APMIS VL - 123 IS - 2 SP - 156 EP - 162 PY - 2015 ER -

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