Chromosome Conformation Capture-Based Dna Hybridization Method for Chromosomal Translocation Screening Publisher Pubmed



Absalan M¹ ; Jabbarpour Z² ; Ghahremani MH³ ; Motevaseli E¹ ; Mahmoudian F¹ ; Karimi R⁴ ; Tavoosidana G¹
Source: Biomarkers in Medicine Published:2023

Abstract

Background: DNA probes have been widely used as diagnostic tools for translocations. This study sought to design a screening tool using ssDNA probes and chromosome conformation capture (3C) library fragment hybridization. Method: The authors focused on developing a probe for the juxtaposed region of MYC and TRD. Fragments of the MYC gene with a thiol modification (MYC-Au NP probe) were functionalized by gold nanoparticles (Au NPs). Then TRD probes were immobilized on a nitrocellulose surface. Hybridization between DNA probes and 3C library fragments of SKW3 cells was determined by color intensity. Results: Optimal hybridization of the 3C library sample of the cell line to probes showed higher color intensity than human umbilical vein endothelial cells. Conclusion: Combining 3C-based techniques and DNA-DNA hybridization can identify rearrangements in cancer cells. © 2023 Future Medicine Ltd.