Down-Expression of Mirna-98 and Over-Expression of Mirna-9 Can Result in Inadequate Immune System Response Against Lung Cancer Publisher



Farzanegan B^{1, 11} ; Bahrami N^{2, 12} ; Birjandi B³ ; Khosravi A⁴ ; Nasab AF⁵ ; Fathi M³ ; Dizaj MKP⁶ ; Daustani M⁷ ; Askari E⁸ ; Mohamadnia A^{8, 9} ; Velayati AA¹⁰
Source: Biointerface Research in Applied Chemistry Published:2021

Abstract

Lung cancer is a disease presenting high incidence and mortality rates. Its most common type is non-small cell lung cancer (NSCLC), constituting about 80-85% of all cases. MicroRNAs (miRNAs) are the molecules playing a role in the regulation of genes. Two crucial cytokines in cancer are interleukin (IL)-10 and IFN-γ. Our objective was to investigate the expression of miRNA-9, miRNA-98, JAK, and STAT3 in patients with NSCLC and detect the cytokine level of IL-10. A total of 50 patients with NSCLC participated in this study. Besides, 50 healthy blood samples were selected as the control group. Real-time PCR determined the expression levels of miRNAs so that the RNAs extracted from the patients' peripheral mononuclear cells (PBMC) were initially synthesized, and cDNA was then extracted. Finally, the synthesized cDNA was amplified using real-time PCR, and its expression was compared to the control group. ELISA technique was used to detect IL-10 in plasma. Our result showed a low level of expression of miRNA-98, JAK, and STAT3 and a high-level expression of miRNA-9. ELISA test indicated a high cytokine level of IL-10 in the NSCLC patients' serum compared to the healthy controls. MiRNA-9 could suppress JAK and STAT3 genes in the NSCLC patients, which causes the poor immune response against the cell's proliferation. Besides, IL-10 was enhanced when miRNA-98 was reduced. Therefore, the elevation of IL-10, as an anti-inflammatory cytokine, could lead to lung cancer progression without a strong immune system response. © 2021 by the authors.