Eliciting Th1 Immune Response Using Casein (Alpha S1)-Loaded Dendritic Cells Pubmed



Daneshmandi S¹ ; Nourizadeh M² ; Pourpak Z² ; Pourfathollah AA¹
Source: Iranian Journal of Allergy# Asthma and Immunology Published:2017

Abstract

Allergen-specific immunotherapy (AIT) has been recently considered as an alternative approach to ameliorate the symptoms of allergen exposure and improvement the patients' quality of life. Dendritic cells (DC) in the forms of tolerogenic or Th1-induced cells have been investigated in several studies as one of the promising approaches of AIT in allergic diseases. The aim of this study was to evaluate the potency of casein-loaded DCs in eliciting the Th1 immune responses in Balb/c mice as a potential therapeutic approach in allergic condition. Immature bone marrow-derived DCs were loaded with casein (protein or mRNA) or green fluorescent protein (GFP) mRNA. DCs were evaluated based on the expression of specific markers and production of proinflammatory cytokines. Proliferation and cytokine production of lymph node lymphocytes and splenocytes were measured in DC-injected mice. Expression of DC markers in all groups was significantly higher than immature DCs, but lower than LPS-Activated DCs. Despite an increase in TNF-α and IL-12, IL-6 was decreased in casein-DC treatments. Casein-loaded DCs could induce proliferation in lymphocytes and stimulate them to produce higher amounts of IFN-γ and in some extent IL-10 and TGF-β, while they could not stimulate IL-4 secretion. Casein-loaded DCs could partially elicit the Th1 responses; this would be a promising approach to use them as an allergic protective way for applying immune cell therapy in cow's milk allergy. Copyright © Spring 2017, Iran J Allergy Asthma Immunol. All rights reserved.