L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity

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L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity Publisher Pubmed

Monajati M^{1, 2} ; Tamaddon AM^{1, 2, 3} ; Abolmaali SS^{1, 2} ; Yousefi G^{2, 3} ; Javanmardi S² ; Borandeh S² ; Heidari R⁴ ; Azarpira N⁵ ; Dinarvand R⁶

Source: Colloids and Surfaces B: Biointerfaces Published:2023

Abstract

L-asparaginase (ASNase) enzyme has limited therapeutic use due to its poor pharmacokinetics and immunogenicity. To overcome these obstacles, we immobilized ASNase in biocompatible poly hydroxypropyl methacrylamide (P(HPMA))-based nanogels simply formed through the host-guest inclusion complex of ASNase-conjugated random copolymer of HPMA and polyethylene glycol (PEG) acrylate (P(HPMA-MPEGA)) and α-cyclodextrin dimer (bisCD) using cystamine as a linker. The effects of bisCD and polymer concentrations on particle size, gelation time, and recovery of enzyme activity were investigated. The ASNase-conjugated bisCD nanogels were discrete, homogeneous, and spherical with a mean projected diameter of 148 ± 41 nm. ASNase immobilized in the bisCD nanogels caused cytotoxicity on HL-60 cell line with IC50 of 3 IU/ml. In-vivo rat study revealed that the immobilized ASNase reduced the enzyme antigenicity and resulted in 8.1 folds longer circulation half-life than the native enzyme. Conclusively, immobilization of ASNase in P(HPMA-MPEGA) and bisCD supramolecular nanogels could enhance the therapeutic value of ASNase in cancer chemotherapy. © 2023

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Style	Citing Format
MLA	Monajati M, et al.. "L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity." Colloids and Surfaces B: Biointerfaces, vol. 225, no. , 2023, pp. -.
APA	Monajati M, Tamaddon AM, Abolmaali SS, Yousefi G, Javanmardi S, Borandeh S, Heidari R, Azarpira N, Dinarvand R (2023). L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity. Colloids and Surfaces B: Biointerfaces, 225(), -.
Chicago	Monajati M, Tamaddon AM, Abolmaali SS, Yousefi G, Javanmardi S, Borandeh S, Heidari R, Azarpira N, Dinarvand R. "L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity." Colloids and Surfaces B: Biointerfaces 225, no. (2023): -.
Harvard	Monajati M et al. (2023) 'L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity', Colloids and Surfaces B: Biointerfaces, 225(), pp. -.
Vancouver	Monajati M, Tamaddon AM, Abolmaali SS, Yousefi G, Javanmardi S, Borandeh S, et al.. L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity. Colloids and Surfaces B: Biointerfaces. 2023;225():-.
BibTex	@article{ author = {Monajati M and Tamaddon AM and Abolmaali SS and Yousefi G and Javanmardi S and Borandeh S and Heidari R and Azarpira N and Dinarvand R}, title = {L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity}, journal = {Colloids and Surfaces B: Biointerfaces}, volume = {225}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Monajati M AU - Tamaddon AM AU - Abolmaali SS AU - Yousefi G AU - Javanmardi S AU - Borandeh S AU - Heidari R AU - Azarpira N AU - Dinarvand R TI - L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity JO - Colloids and Surfaces B: Biointerfaces VL - 225 IS - SP - EP - PY - 2023 ER -

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