Low-Voltage Online Stimulated Microextraction of Glibenclamide From Whole Blood Publisher



Norouzi P^{1, 2} ; Rezaei Akmal M¹ ; Mofidi Z¹ ; Larijani B³ ; Ganjali MR^{1, 2} ; Ebrahimi M¹
Source: Microchemical Journal Published:2019

Abstract

In the present study, Glibenclamide (GLI)was determined in blood samples through the application of a method based on coupling low-voltage electromembrane extraction (LV-EME)and fast Fourier transform square wave voltammetry (FFTSWV). The parameters influencing the LV-EME of GLI (i.e. extraction time, electrical potential and pH of donor phases)were optimized using a central composite design (CCD)and the optimal values of the important parameters for the LV-EME-FFTSWV were obtained to be (frequency = 1420 Hz/amplitude = 20 mV, extraction time = 30 min, applied voltage = 5 V, supported liquid membrane (SLM)= 1-octanol, pH values of the feed and receive sources = 8.0 and 7.0). Under these conditions, GLI was extracted from blood samples with a recovery percentage of 26. The LV-EME-FFTSWV method was found to have linear responses over two wide concentration ranges of 10–1000 and 1000–10,000 ng/mL and determination coefficients of 0.997 and 0.994, respectively. The limits of detection and quantification of 3 ng/mL and 10 ng/mL and also the intra- and inter-day precisions of 3.8% and 5.1% were determined. © 2019