Quantitative Polymerase Chain Reaction for Detection of Human Herpesvirus-7 Infection in Umbilical Cord Blood Donors Publisher Pubmed



Abedi E¹ ; Kheirandish M¹ ; Sharifi Z² ; Samiee S³ ; Kokhaei P⁴ ; Pourpak Z⁵ ; Ashraf MJ⁶
Source: Transplant Infectious Disease Published:2015

Abstract

Objective: Umbilical cord blood (UCB) has been a reasonable alternative to granulocyte colony-stimulating factor-mobilized peripheral blood or bone marrow, as a source of hematopoietic stem cells with a lower risk of graft-versus-host disease. In immunocompromised hosts after transplantation, the risk of viral infection in adults, especially with beta-herpesviruses such as human herpesvirus-7 (HHV-7), may be increased. This virus in immunocompromised patients can be reactivated from latency and converted to an active phase. Therefore, light-upon-extension real-time polymerase chain reaction (PCR) was developed to assess the prevalence and load of HHV-7 in the plasma and buffy coat of donors. Methods: About 825 UCB samples under standard protocol from donors were collected. Then, DNA from plasma and buffy coat was extracted and quantitative real-time PCR was performed with light-upon-extension primers. Results: Overall, HHV-7 was detected in 3.64% (30/825) of UCB donors. HHV-7 DNA was detected in 26 (3.2%) buffy coat samples (latent infection), and only 4 (0.48%) of them were positive for HHV-7 DNA in plasma samples (active infection); the mean HHV-7 viral load was 1.31 × 101 copies/mL in latent infection, and 1.94 × 105 copies/mL in active infection. Conclusions: We suggest that real-time PCR in plasma and buffy coat could be a useful method to detect active and latent HHV-7 infection in UCB donors and determine its role in subsequent transmission events. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.