Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
The Effect of the Carbodiimide Cross-Linker on the Structural and Biocompatibility Properties of Collagen–Chondroitin Sulfate Electrospun Mat Publisher Pubmed



Akhshabi S1 ; Biazar E2 ; Singh V3 ; Keshel SH4 ; Geetha N1
Authors

Source: International Journal of Nanomedicine Published:2018


Abstract

Background: Collagen and chondroitin sulfate (CS) are an essential component of the natural extracellular matrix (ECM) of most tissues. They provide the mechanical stability to cone the compressive forces in ECM. In tissue engineering, electrospun nanofibrous scaffolds prepared by electrospinning technique have emerged as a suitable candidate to imitate natural ECM functions. Cross-linking with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/ N-hydroxy succinimide can overcome the weak mechanical integrity of the engineered scaffolds in addition to the increased degradation stability under physiological conditions. Materials and methods: This study has synthesized nanofibrous collagen–CS scaffolds by using the electrospinning method. Results: The results have shown that incorporation of CS in higher concentration, along with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/N-hydroxy succinimide, enhanced mechanical stability. Scaffolds showed more resistance to collagenase digestion. Fabricated scaffolds showed biocompatibility in corneal epithelial cell attachment. Conclusion: These results demonstrate that cross-linked electrospun CO–CS mats exhibited a uniform nanofibrous and porous structure, especially for lower concentration of the cross-linker and may be utilized as an alternative effective substrate in tissue engineering. © 2018 Akhshabi et al.
Other Related Docs
14. Hybrid Electrospun Scaffold Loaded With Argireline Acetate and Dexpanthenol for Skin Regeneration, International Journal of Polymeric Materials and Polymeric Biomaterials (2023)